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New articles referring Doccol from January to September in 2018
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Modeling Transient Focal Ischemic Strokein Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

MS Lopez, R Vemuganti - Traumatic and Ischemic Injury, 2018 - Springer
… Middle cerebral artery occlusion (MCAO) has been a valuable model of focal ischemic stroke since 1981, when Tamura et al. [1] demonstrated permanent occlusion in the distal MCA via craniectomy … (18a) MCAO filaments for mice (Doccol, #6023) …
Related articles All 4 versions

MRI in the Study of Animal Models of stroke

P Ramos-Cabrer, D Padro - Preclinical MRI, 2018 - Springer
… 3.1 stroke Model: Occlusion of the Medial Cerebral Artery (MCAo) … A nylon filament with a rounded silicone tip may do the job, but we strongly recommend commercial suture L34 PK10 from Doccol—see materials section—to increase success rate and reproducibility) is …
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Reducing myeloperoxidase activity decreases inflammation and increases cellular protection in ischemic stroke

HJ Kim, Y Wei, GR Wojtkiewicz… - Journal of Cerebral …, 2018 - journals.sagepub.com
Myeloperoxidase (MPO) is a pro-inflammatory enzyme abundantly secreted by activated myeloid cells after stroke. We show that when MPO activity is either blocked... 
A small nick was made in the ECA stump and a 7-0 fine surgical nylon monofilament (diameter
0.19-0.20 mm) coated with silicon (Cat # 7019PK5Re,Doccol Co., Sharon, MA, USA) was
inserted into the right ICA lumen through the ECA stump and advanced up to the MCA origin at
the Circle of Willis.
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[HTML] Immunomodulatory treatment with systemic GM-CSF augments pulmonary immune responses and improves neurological outcome after experimental stroke

C Dames, K Winek, Y Beckers, O Engel… - Journal of …, 2018 - Elsevier
… and water. 2.2. Experimental stroke (middle cerebral artery occlusion, MCAo). The middle cerebral … Berlin (Dirnagl, 2010). In brief, after ventral cervical incision a silicon-coated filament (7019PK5Re, Doccol Corp. Redlands, California …
Cited by 1 Related articles All 6 versions

[HTML] Vitamin D3 Supplementation Reduces Subsequent Brain Injury and Inflammation Associated with Ischemic stroke

MA Evans, HA Kim, YH Ling, S Uong, A Vinh… - Neuromolecular …, 2018 - Springer
… Animals were injected ip for 5 consecutive days prior to experimental stroke and again on the day of the procedure, as previously described … xylazine: 10 mg/kg ip) by occlusion of the middle cerebral artery (MCA) using a 6.0 silicone-coated monofilament (Doccol Corporation) …
Cited by 2 Related articles All 5 versions

Ischemic Strokedetection by analyzing heart rate variability in rat middle cerebral artery occlusion model

T Kodama, K Kamata, K Fujiwara… - … on Neural Systems …, 2018 - ieeexplore.ieee.org
… to the common carotid artery (CCA) and a nylon monofilament coated with silicon resin (5-0 large MCAO suture B3 L23 PK10, Doccol Corporation) is inserted through ECA to occlude the right MCA. It is important to investigate the area and volume of ischemic stroke in the brain …
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[HTML] Myeloid-specific TAK1 deletion results in reduced brain monocyte infiltration and improved outcomes after stroke

A Chauhan, J Hudobenko… - Journal of …, 2018 - jneuroinflammation.biomedcentral …
… A midline ventral neck incision was made, and unilateral MCAO was performed by inserting a 6.0 Doccol monofilament (Doccol Corp, Redlands, CA, USA) into the right internal carotid … Following reperfusion, mice were sacrificed at day 3 after stroke …
Related articles All 7 versions

T Cells Prevent Hemorrhagic Transformation in Ischemic Strokeby P-Selectin Binding

A Salas-Perdomo, F Miró-Mur, X Urra… - … , and vascular biology, 2018 - Am Heart Assoc
… 38, No. 8T Cells Prevent Hemorrhagic Transformation in Ischemic stroke by P-Selectin Binding. No Accessarticle Download PDF … Supplementary Materials. No Accessarticle Download PDF. T Cells Prevent Hemorrhagic Transformation in Ischemic stroke by P-Selectin Binding …
Occlusion of the right middle cerebral artery (MCAo) was performed under isoflurane anesthesia using a monofilament (no. 701912PK5Re, Doccol Corporation, Sharon, MA), ...
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AKAP1 protects from cerebral ischemic Strokeby inhibiting Drp1-dependent mitochondrial fission

KH Flippo, A Gnanasekaran, GA Perkins… - Journal of …, 2018 - Soc Neuroscience
… We found that AKAP1 loss worsens 101 outcomes of transient MCAO, a model of focal ischemic stroke. Indicative of Drp1 hyperactivation, 102 … siliconized filament (Doccol Corp.) followed by 23 hours of reperfusion. Body temperature was 126 …
All 5 versions

SIRT2 Inhibition Confers Neuroprotection by Downregulation of FOXO3a and MAPK Signaling Pathways in Ischemic stroke

DT She, LJ Wong, SH Baik, TV Arumugam - Molecular neurobiology, 2018 - Springer
… and non-histones pro- teins, and modulations of sirtuins have been showed to signif- icantly dictate the outcome of ischemic stroke [11] … was cut and a 6-0 monofilament with the tip blunted and coated with silicone rubber (0.21–0.23 mm in diameter, Doccol Corporation, Sharon …
Cited by 2 Related articles All 3 versions

Impaired Neurofilament Integrity and Neuronal Morphology in Different Models of Focal Cerebral Ischemia and Human StrokeTissue

B Mages, S Aleithe, S Altmann, A Blietz… - Frontiers in cellular …, 2018 - frontiersin.org
… Further details on the analyzed brain tissue and stroke characterization are given in Kattah et al … 33 mg/kg body weight, Hypnomidate, Janssen-Cilag, Neuss, Germany) followed by insertion of a standardized silicon-coated 6-0 monofilament (Doccol Corporation, Redlands, CA …
All 2 versions

Stepwise impairment of neural stem cell proliferation and neurogenesis concomitant with disruption of blood-brain barrier in recurrent ischemic stroke

R Lin, M Lang, N Heinsinger, G Stricsek, J Zhang… - Neurobiology of …, 2018 - Elsevier
… For the first stroke (MCAO), the rats were placed supine after anesthesia and MCAO was performed using a slight modification of our … Briefly, the right common carotid (CCA) was temporally ligated and a silicone rubber-coated nylon filament (Doccol) was inserted into the ECA …
Cited by 2 Related articles All 3 versions

[HTML] Protective Effects of L-902,688, a Prostanoid EP4 Receptor Agonist, against Acute Blood-Brain Barrier Damage in Experimental Ischemic stroke

KM DeMars, AO McCrea, DM Siwarski… - Frontiers in …, 2018 - frontiersin.org
… To mimic ischemic stroke, rats were subjected to 90 min of transient middle cerebral artery occlusion (MCAO) using the intraluminal filament … the CCA a few millimeters above the ligation to allow for a 4-0 silicone-coated filament (Cat # 403523PK10; Doccol Corporation, Sharon …
Related articles All 5 versions

[HTML] CNS-border associated macrophages respond to acute ischemic Strokeattracting granulocytes and promoting vascular leakage

J Pedragosa, A Salas-Perdomo… - Acta …, 2018 - actaneurocomms.biomedcentral …
… damage and triggers sterile inflammation and innate immune responses [5, 26] that are considered as putative targets for therapeutic intervention [4]. While the responses of microglia to ischemic stroke have been … The MCA was occluded with a filament (Doccol #403912PK10Re …
All 7 versions

Apixaban decreases brain thrombin activity in a male mouse model of acute ischemic stroke

D Bushi, J Chapman, A Wohl, ES Stein… - Journal of …, 2018 - Wiley Online Library
… 2.2 Effect of apixaban on brain thrombin activity and infarct volume following focal ischemic stroke … Permanent MCA occlusion (pMCAo) was performed as previously reported by our lab (Bushi et al., 2013, 2015, 2016) using silicone‐coated filament (Doccol Corp, Redlands, CA …
Related articles All 3 versions

Acute mitogen‐activated protein kinase 1/2 inhibition improves functional recovery and vascular changes after ischaemic Strokein rat‐monitored by 9.4 T magnetic …

M Mostajeran, F Wetterling, F W. Blixt… - Acta …, 2018 - Wiley Online Library
… potential in the future because it clearly shows a beneficial effect after stroke even when administrated 6 hours after stroke onset … A silicon rubber‐coated monofilament (Doccol Corporation, MA, USA) was inserted into the right internal carotid artery and further advanced until the …
Cited by 1 Related articles All 6 versions

Selective role of Na+/H+ exchanger in Cx3cr1+ microglial activation, white matter demyelination, and post‐Strokefunction recovery

S Song, S Wang, VM Pigott, T Jiang, LM Foley… - Glia, 2018 - Wiley Online Library
Silicone‐coated suture was from Doccol (Redlands, CA, USA) … Krieger, Tetzlaff, & Rossi, 2007; Fogg et al., 2006; Valny, Honsa, Kirdajova, Kamenik, & Anderova, 2016) and for replenishing of Cx3cr1 + monocytes (Parkhurst et al., 2013) prior to induction of ischemic stroke …
All 4 versions

Beta adrenoceptor blockade ameliorates impaired glucose tolerance and alterations of the cerebral ceramide metabolism in an experimental model of ischemic stroke

S Luger, A Schwebler, R Vutukuri… - Therapeutic …, 2018 - journals.sagepub.com
Background: Sphingolipids are versatile signaling molecules derived from membrane lipids of eukaryotic cells. Ceramides regulate cellular processes such as prol...
Focal cerebral ischemia was induced after a midline cervical incision and exposure of the right carotid bifurcation by introducing a standardized silicone-coated monofilament with a tip diameter of 0.23 mm (Doccol, Redlands, CA, USA) and advancing it along the internal carotid artery until the tip occluded the proximal stem of the middle cerebral artery.
Related articles All 5 versions

[HTML] Inhibition of the NLRP3-inflammasome as a potential approach for neuroprotection after stroke

S Ismael, L Zhao, S Nasoohi, T Ishrat - Scientific reports, 2018 - nature.com
Article | Open. Inhibition of the NLRP3-inflammasome as a potential approach for neuroprotection after stroke … animals. Full size image. MCC950 treatment prevents stroke-induced NLRP3 inflammasome activation. The principal …
Middle cerebral artery occlusion (MCAO) was achieved using a 6–0 silicon-coated nylon suture (Doccol, Sharon, MA), advanced into the internal carotid artery to block the origin of the middle cerebral artery.
Cited by 3 Related articles All 8 versions

Vascular endothelial growth factor isoform-B stimulates neurovascular repair
after ischemic Strokeby promoting the function of pericytes via vascular endothelial …

NJ LeBlanc, R Guruswamy, A ElAli - Molecular neurobiology, 2018 - Springer
… Adult C57BL6/j male mice (3 months old) were subjected to focal ischemic stroke via the transient occlusion of
the MCA using an … A microvascular clip was placed on the internal carotid artery, and a 7–0 silicon-coated nylon
monofilament
(Doccol Corporation, MA, USA) was …

Cited by 9 Related articles All 3 versions

The combination of mannitol and temozolomide increases the effectiveness of stem cell treatment in a chronic Strokemodel

C Choi, HM Kim, J Shon, J Park, HT Kim… - …, 2018 - celltherapyjournal.org
… The combination of mannitol and temozolomide increases the effectiveness of stem cell treatment in a chronic stroke model … Email the author Ok Joon Kim. The combination of mannitol and temozolomide increases the effectiveness of stem cell treatment in a chronic stroke model …
The external branch of the carotid artery (ECA) was punctured and filaments (Doccol Corp.) were inserted to block the middle cerebral artery. After 60 min, the ...
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Plasma Unesterified Fatty‐Acid Profile Is Dramatically and Acutely Changed under Ischemic Strokein the Mouse Model

SA Golovko, MY Golovko - Lipids, 2018 - Wiley Online Library
… These results are the first to identify plasma FFA pro- files as a potential biomarker for early ischemic stroke within the … Briefly, mice were anesthetized with 1.75–2.25% isoflurane, and a standard- ized microfilament (702345PK5Re, Doccol Corporation, Sharon, MA, USA) was …
All 3 versions

KLF4 protects brain microvascular endothelial cells from ischemic strokeinduced apoptosis by transcriptionally activating MALAT1

H Yang, X Xi, B Zhao, Z Su, Z Wang - Biochemical and biophysical …, 2018 - Elsevier
… long noncoding RNA (LncRNA), might be key mediators of posttranscriptional gene expression/function in pathology of ischemic stroke [7] ; [8 … produced by intra-luminal occlusion of the right MCA using a silicone coated nylon (6-0) monofilament (Doccol Corporation, Redlands …
Cited by 3 Related articles All 4 versions

[HTML] strokeinfarct volume estimation in fixed tissue: Comparison of diffusion kurtosis imaging to diffusion weighted imaging and histology in a rodent MCAO model

V Bay, BF Kjølby, NK Iversen, IK Mikkelsen, M Ardalan… - PloS one, 2018 - journals.plos.org
… The fast kurtosis techniques have been validated against conventional DKI in an animal model of stroke [36] and have been shown to be contrast-to-noise efficient compared to conventional DKI [30] … [39] using a 4–0 reusable suture (4041PK5Re, Doccol Corporation, Sharon …
Related articles All 9 versions

[HTML] PAI-1 but Not PAI-2 Gene Deficiency Attenuates Ischemic Brain Injury After Experimental stroke

EV Griemert, KR Pelz, K Engelhard, MK Schäfer… - Translational stroke …, 2018 - Springer
… First, the time-dependent regulation of plasminogen activator system genes after stroke was exam- ined by quantitative PCR (qPCR) and then the … Brain ischemia was induced for 60 min by temporary MCAO using a silicone-coated 6–0 filament (6023; Doccol Corp., Redlands …
All 4 versions

A deficiency of the GluN2C subunit of the N-methyl-D-aspartate receptor is neuroprotective in a mouse model of ischemic stroke

A Holmes, N Zhou, DL Donahue, R Balsara… - Biochemical and …, 2018 - Elsevier
… A coated-tip suture (#602334, Doccol Corp., Sharon, MA) was inserted to occlude the middle carotid artery via the external carotid artery … brain specimens for analysis: (1) death before 24áh post-MCAO time point; (2) absence of any detectable ischemic infarct; (3) stroke in the …
Related articles All 4 versions

Time-of-day dependent neuronal injury after ischemic stroke: implication of circadian clock transcriptional factor bmal1 and survival kinase AKT

MC Beker, B Caglayan, E Yalcin, AB Caglayan… - Molecular …, 2018 - Springer
… A 7-0 silicon-coated nylon monofilament (701934PK5Re, Doccol; USA) was inserted through a small incision into the common carotid artery … For 30 min MCAo, tissue samples obtained from the ipsilateral to the stroke were collected for proteomics, planar surface immunoassay …
Cited by 7 Related articles All 3 versions

[HTML] Experimental Strokedifferentially affects discrete subpopulations of splenic macrophages

L McCulloch, A Alfieri, BW McColl - Frontiers in immunology, 2018 - ncbi.nlm.nih.gov
… Experimental stroke Model and Tissue Harvesting. Middle cerebral artery occlusion (MCAO) was performed under isoflourane anesthesia (with O 2 and N 2 O) by insertion of a 6-0 nylon monofilament with a 2 mm coated tip (210 µm diameter; Doccol, USA) through the external …
Related articles All 7 versions

Quantitative assessment of demyelination in ischemic Stroke in vivo using macromolecular proton fraction mapping

MY Khodanovich, AA Kisel, AE Akulov… - Journal of Cerebral …, 2018 - journals.sagepub.com
A recent MRI method, fast macromolecular proton fraction (MPF) mapping, was used to quantify demyelination in the transient middle cerebral artery occlusion (MC...
Transient left МСАО was achieved in 15 animals by inserting a nylon filament (diameter 0.185 mm, length 30 mm, diameter with coating 0.39 ± 0.02 mm, coating length 9–10 mm; Doccol, USA) to the origin of the artery for 1 h as described previously
Related articles All 3 versions

Sequential combined Treatment of Pifithrin-α and Posiphen Enhances Neurogenesis and Functional Recovery After stroke

F Turcato, P Kim, A Barnett, Y Jin… - Cell …, 2018 - journals.sagepub.com
Objective: Although cerebral ischemia can activate endogenous reparative processes, such as proliferation of endogenous neural stem cells (NSCs) in the subventr...
A nylon filament suture with a silicon coated tip (Doccol Co., Sharon, MA,  USA)  was  inserted  into  the  lECA  stump  and  further pushed along the lumen of the internal carotid artery while the clamp was released, and then the tie around lICA was tightened....
Related articles All 3 versions

Neuronal PirB upregulated in cerebral ischemia acts as an attractive theranostic target for ischemic stroke

J Wang, Y Zhang, J Xia, T Cai, J Du… - Journal of the …, 2018 - Am Heart Assoc
… probes for real‐time monitoring of ischemic stroke, and to deliver soluble PirB ectodomains by nanoliposomes to improve stroke model recovery … A 6‐0 nylon monofilament suture (Doccol Co., Redlands, CA) was inserted from the common carotid artery into the internal carotid …
Cited by 1 Related articles All 6 versions

Age of donor of human mesenchymal stem cells affects structural and functional recovery after cell therapy following ischaemic stroke

S Yamaguchi, N Horie, K Satoh… - Journal of Cerebral …, 2018 - journals.sagepub.com
Cell transplantation therapy offers great potential to improve impairments after stroke. However, the importance of donor age on therapeutic efficacy is unclear...
Transient middle cerebral artery occlusion was performed for 75 min with 4.0 nylon monofilament sutures coated with silicone (4039PK10; Doccol) as previously described...
Cited by 2 Related articles All 6 versions

Heat shock protein A12A encodes a novel prosurvival pathway during ischaemic stroke

Y Mao, Q Kong, R Li, X Zhang, Y Gui, Y Li, C Li… - … et Biophysica Acta (BBA …, 2018 - Elsevier
… Ischaemic stroke was induced by left middle cerebral artery occlusion (MCAO) for 1 h followed by blood reperfusion as we previously described … A 6–0 filament coated with silicon resin (Doccol Corp., Sharon, MA) was introduced into the left common carotid artery and advanced …
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[HTML] L-3-n-Butylphthalide reduces ischemic Strokeinjury and increases M2 microglial polarization

F Li, Q Ma, H Zhao, R Wang, Z Tao, Z Fan… - Metabolic brain …, 2018 - Springer
… of NBP on microglial polarization and inflammatory responses in the middle cerebral artery occlusion (MCAO) model of ischemic stroke … A silicone rubber-coated monofilament su- ture (Catalogue number: 701956PK5Re; Doccol Corporation, Sharon, MA, USA) was inserted into …
All 3 versions

Role of Steroid Therapy after Ischemic Strokeby n-Methyl-d-Aspartate Receptor Gene Regulation

AA Tameh, M Karimian, Z Zare-Dehghanani… - Journal of Strokeand …, 2018 - Elsevier
… Transient middle cerebral artery occlusion (tMCAO) was used to prepare experimental stroke as described previously … Then a silicon-coated monofilament (404356PK10Re, Doccol, Sharon, Massachusetts) introduced into the lumen of CCA and forwarded to internal carotid …
All 5 versions

High intensity exercise preconditioning provides differential protection against brain injury following experimental stroke

R Rezaei, S Nasoohi, A Haghparast, F Khodagholi… - Life sciences, 2018 - Elsevier
… Intraluminal middle cerebral artery occlusion (MCAO) was applied to induce ischemic stroke, as described previously [26]. Briefly, animals were anesthetized with chloral hydrate (400 mg/kg, ip) and a 4–0 silicon-coated nylon suture (OD: 0.35 mm; Doccol, USA) was advanced …
Related articles All 3 versions

[HTML] Transcriptomic analysis of neuregulin-1 regulated genes following ischemic strokeby computational identification of promoter binding sites: A role for the ETS …

MC Surles-Zeigler, Y Li, TJ Distel, H Omotayo, S Ge… - PloS one, 2018 - journals.plos.org
… These findings could support the development of clinical studies using NRG1 for the treatment of patients with acute stroke … Briefly, a 4 cm length 4–0 surgical monofilament nylon suture coated with silicon (Doccol Corp., Sharon, MA, diameter 0.37 mm, length 2.3–2.5 mm) was …
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