… Middle cerebral artery occlusion (MCAO) has been a valuable model of focal ischemic stroke since 1981, when Tamura et al. [1] demonstrated permanent occlusion in the distal MCA via craniectomy … (18a) MCAO filaments for mice (Doccol, #6023) …

… 3.1 stroke Model: Occlusion of the Medial Cerebral Artery (MCAo) … A nylon filament with a rounded silicone tip may do the job, but we strongly recommend commercial suture L34 PK10 from Doccol—see materials section—to increase success rate and reproducibility) is …

Myeloperoxidase (MPO) is a pro-inflammatory enzyme abundantly secreted by activated myeloid cells after stroke. We show that when MPO activity is either blocked... 

… and water. 2.2. Experimental stroke (middle cerebral artery occlusion, MCAo). The middle cerebral … Berlin (Dirnagl, 2010). In brief, after ventral cervical incision a silicon-coated filament (7019PK5Re, Doccol Corp. Redlands, California …

… Animals were injected ip for 5 consecutive days prior to experimental stroke and again on the day of the procedure, as previously described … xylazine: 10 mg/kg ip) by occlusion of the middle cerebral artery (MCA) using a 6.0 silicone-coated monofilament (Doccol Corporation) …

… to the common carotid artery (CCA) and a nylon monofilament coated with silicon resin (5-0 large MCAO suture B3 L23 PK10, Doccol Corporation) is inserted through ECA to occlude the right MCA. It is important to investigate the area and volume of ischemic stroke in the brain …

… A midline ventral neck incision was made, and unilateral MCAO was performed by inserting a 6.0 Doccol monofilament (Doccol Corp, Redlands, CA, USA) into the right internal carotid … Following reperfusion, mice were sacrificed at day 3 after stroke …

… 38, No. 8T Cells Prevent Hemorrhagic Transformation in Ischemic stroke by P-Selectin Binding. No Accessarticle Download PDF … Supplementary Materials. No Accessarticle Download PDF. T Cells Prevent Hemorrhagic Transformation in Ischemic stroke by P-Selectin Binding …

… We found that AKAP1 loss worsens 101 outcomes of transient MCAO, a model of focal ischemic stroke. Indicative of Drp1 hyperactivation, 102 … siliconized filament (Doccol Corp.) followed by 23 hours of reperfusion. Body temperature was 126 …

… and non-histones pro- teins, and modulations of sirtuins have been showed to signif- icantly dictate the outcome of ischemic stroke [11] … was cut and a 6-0 monofilament with the tip blunted and coated with silicone rubber (0.21–0.23 mm in diameter, Doccol Corporation, Sharon …

… Further details on the analyzed brain tissue and stroke characterization are given in Kattah et al … 33 mg/kg body weight, Hypnomidate, Janssen-Cilag, Neuss, Germany) followed by insertion of a standardized silicon-coated 6-0 monofilament (Doccol Corporation, Redlands, CA …

… For the first stroke (MCAO), the rats were placed supine after anesthesia and MCAO was performed using a slight modification of our … Briefly, the right common carotid (CCA) was temporally ligated and a silicone rubber-coated nylon filament (Doccol) was inserted into the ECA …

… To mimic ischemic stroke, rats were subjected to 90 min of transient middle cerebral artery occlusion (MCAO) using the intraluminal filament … the CCA a few millimeters above the ligation to allow for a 4-0 silicone-coated filament (Cat # 403523PK10; Doccol Corporation, Sharon …

… damage and triggers sterile inflammation and innate immune responses [5, 26] that are considered as putative targets for therapeutic intervention [4]. While the responses of microglia to ischemic stroke have been … The MCA was occluded with a filament (Doccol #403912PK10Re …

… 2.2 Effect of apixaban on brain thrombin activity and infarct volume following focal ischemic stroke … Permanent MCA occlusion (pMCAo) was performed as previously reported by our lab (Bushi et al., 2013, 2015, 2016) using silicone‐coated filament (Doccol Corp, Redlands, CA …

… potential in the future because it clearly shows a beneficial effect after stroke even when administrated 6 hours after stroke onset … A silicon rubber‐coated monofilament (Doccol Corporation, MA, USA) was inserted into the right internal carotid artery and further advanced until the …

… Silicone‐coated suture was from Doccol (Redlands, CA, USA) … Krieger, Tetzlaff, & Rossi, 2007; Fogg et al., 2006; Valny, Honsa, Kirdajova, Kamenik, & Anderova, 2016) and for replenishing of Cx3cr1 + monocytes (Parkhurst et al., 2013) prior to induction of ischemic stroke …

Background: Sphingolipids are versatile signaling molecules derived from membrane lipids of eukaryotic cells. Ceramides regulate cellular processes such as prol...
Focal cerebral ischemia was induced after a midline cervical incision and exposure of the right carotid bifurcation by introducing a standardized silicone-coated monofilament with a tip diameter of 0.23 mm (Doccol, Redlands, CA, USA) and advancing it along the internal carotid artery until the tip occluded the proximal stem of the middle cerebral artery.

Article | Open. Inhibition of the NLRP3-inflammasome as a potential approach for neuroprotection after stroke … animals. Full size image. MCC950 treatment prevents stroke-induced NLRP3 inflammasome activation. The principal …
Middle cerebral artery occlusion (MCAO) was achieved using a 6–0 silicon-coated nylon suture (Doccol, Sharon, MA), advanced into the internal carotid artery to block the origin of the middle cerebral artery.

… The combination of mannitol and temozolomide increases the effectiveness of stem cell treatment in a chronic stroke model … Email the author Ok Joon Kim. The combination of mannitol and temozolomide increases the effectiveness of stem cell treatment in a chronic stroke model …
The external branch of the carotid artery (ECA) was punctured and filaments (Doccol Corp.) were inserted to block the middle cerebral artery. After 60 min, the ...

… These results are the first to identify plasma FFA pro- files as a potential biomarker for early ischemic stroke within the … Briefly, mice were anesthetized with 1.75–2.25% isoflurane, and a standard- ized microfilament (702345PK5Re, Doccol Corporation, Sharon, MA, USA) was …

… long noncoding RNA (LncRNA), might be key mediators of posttranscriptional gene expression/function in pathology of ischemic stroke [7] ; [8 … produced by intra-luminal occlusion of the right MCA using a silicone coated nylon (6-0) monofilament (Doccol Corporation, Redlands …

… The fast kurtosis techniques have been validated against conventional DKI in an animal model of stroke [36] and have been shown to be contrast-to-noise efficient compared to conventional DKI [30] … [39] using a 4–0 reusable suture (4041PK5Re, Doccol Corporation, Sharon …

… First, the time-dependent regulation of plasminogen activator system genes after stroke was exam- ined by quantitative PCR (qPCR) and then the … Brain ischemia was induced for 60 min by temporary MCAO using a silicone-coated 6–0 filament (6023; Doccol Corp., Redlands …

… A coated-tip suture (#602334, Doccol Corp., Sharon, MA) was inserted to occlude the middle carotid artery via the external carotid artery … brain specimens for analysis: (1) death before 24áh post-MCAO time point; (2) absence of any detectable ischemic infarct; (3) stroke in the …

… A 7-0 silicon-coated nylon monofilament (701934PK5Re, Doccol; USA) was inserted through a small incision into the common carotid artery … For 30 min MCAo, tissue samples obtained from the ipsilateral to the stroke were collected for proteomics, planar surface immunoassay …

… Experimental stroke Model and Tissue Harvesting. Middle cerebral artery occlusion (MCAO) was performed under isoflourane anesthesia (with O 2 and N 2 O) by insertion of a 6-0 nylon monofilament with a 2 mm coated tip (210 µm diameter; Doccol, USA) through the external …

A recent MRI method, fast macromolecular proton fraction (MPF) mapping, was used to quantify demyelination in the transient middle cerebral artery occlusion (MC...
Transient left МСАО was achieved in 15 animals by inserting a nylon filament (diameter 0.185 mm, length 30 mm, diameter with coating 0.39 ± 0.02 mm, coating length 9–10 mm; Doccol, USA) to the origin of the artery for 1 h as described previously

Objective: Although cerebral ischemia can activate endogenous reparative processes, such as proliferation of endogenous neural stem cells (NSCs) in the subventr...
A nylon filament suture with a silicon coated tip (Doccol Co., Sharon, MA,  USA)  was  inserted  into  the  lECA  stump  and  further pushed along the lumen of the internal carotid artery while the clamp was released, and then the tie around lICA was tightened....

… probes for real‐time monitoring of ischemic stroke, and to deliver soluble PirB ectodomains by nanoliposomes to improve stroke model recovery … A 6‐0 nylon monofilament suture (Doccol Co., Redlands, CA) was inserted from the common carotid artery into the internal carotid …

Cell transplantation therapy offers great potential to improve impairments after stroke. However, the importance of donor age on therapeutic efficacy is unclear...
Transient middle cerebral artery occlusion was performed for 75 min with 4.0 nylon monofilament sutures coated with silicone (4039PK10; Doccol) as previously described...

… Ischaemic stroke was induced by left middle cerebral artery occlusion (MCAO) for 1 h followed by blood reperfusion as we previously described … A 6–0 filament coated with silicon resin (Doccol Corp., Sharon, MA) was introduced into the left common carotid artery and advanced …

… of NBP on microglial polarization and inflammatory responses in the middle cerebral artery occlusion (MCAO) model of ischemic stroke … A silicone rubber-coated monofilament su- ture (Catalogue number: 701956PK5Re; Doccol Corporation, Sharon, MA, USA) was inserted into …

… Transient middle cerebral artery occlusion (tMCAO) was used to prepare experimental stroke as described previously … Then a silicon-coated monofilament (404356PK10Re, Doccol, Sharon, Massachusetts) introduced into the lumen of CCA and forwarded to internal carotid …

… Intraluminal middle cerebral artery occlusion (MCAO) was applied to induce ischemic stroke, as described previously [26]. Briefly, animals were anesthetized with chloral hydrate (400 mg/kg, ip) and a 4–0 silicon-coated nylon suture (OD: 0.35 mm; Doccol, USA) was advanced …

… These findings could support the development of clinical studies using NRG1 for the treatment of patients with acute stroke … Briefly, a 4 cm length 4–0 surgical monofilament nylon suture coated with silicon (Doccol Corp., Sharon, MA, diameter 0.37 mm, length 2.3–2.5 mm) was …