Doccol has been serving the stroke research field by providing MCAO sutures (silicone rubber-coated monofilaments) since 2004. Our delicate silicone rubber-coated monofilaments are specially designed and manufactured for inducing the intraluminal filament model of middle cerebral artery occlusion (MCAO), which has been widely used for in vivo experimental stroke studies. Using Doccol MCAO sutures for inducing stroke models will improve your model quality by reducing the occurrence of both the incomplete occlusion and the intracranial bleeding. With Doccol MCAO sutures, you will be able to achieve a highly consistent infarct as well as a low mortality rate. In addition to the super product quality, Doccol provides MCAO sutures with a great variety that was designed to meet the diverse demand from stroke investigators. For many years our MCAO sutures have been preferably selected by stroke investigators for inducing stroke models. We are proud to say that Our Customers are top-ranked universities, highly prestigious research institutes, and world-leading pharmaceutical companies from the United States, Canada, most European countries, Australia, Japan, South Korea, and India. Using Doccol MCAO sutures for inducing MCAO models has been reported to be able to reduce the standard deviation to 5% of its corresponding mean value as for infarction volume; and this modeling method is becoming a new standard for ensuring the highest quality of MCAO models. For more information please read Doccol Made a Difference in Stroke Modeling.
The following are selected publications that refer to our products:
A Comparative Study of Variables Influencing Ischemic Injury in the Longa and Koizumi Methods of Intraluminal Filament Middle Cerebral Artery Occlusion in Mice. PLoS One. 2016 Feb 12;11(2):e0148503. doi: 10.1371/journal.pone.0148503. PMID: 26870954
Uluç K, Miranpuri A, Kujoth GC, Aktüre E, Başkaya MK. (Video Aricle)Focal cerebral ischemia model by endovascular suture occlusion of the middle cerebral artery in the rat. J Vis Exp. 2011 Feb 5;(48). pii: 1978. doi: 10.3791/1978. PMID: 21339721Popp A, Jaenisch N, Witte OW, Frahm C.Identification of ischemic regions in a rat model of stroke. PLoS One. 2009;4(3):e4764. Epub 2009 Mar 10. PMID: 19274095 Lang D, Ude C, Wurglics M, Schubert-Zsilavecz M, Klein J. Brain permeability of bilobalide as probed by microdialysis before and after middle cerebral artery occlusion in mice. J Pharm Pharm Sci. 2010;13(4):607-14. PMID: 21486534Hoda MN, Singh I, Singh AK, Khan M. Reduction of lipoxidative load by secretory phospholipase A2 inhibition protects against neurovascular injury following experimental stroke in rat. J Neuroinflammation. 2009 Aug 13;6:21. PMID: 19678934Liu F, Schafer DP, McCullough LD. TTC, fluoro-Jade B and NeuN staining confirm evolving phases of infarction induced by middle cerebral artery occlusion. J Neurosci Methods. 2009 Apr 30;179(1):1-8. Epub 2009 Jan 9. PMID: 19167427
Kim E, Tolhurst AT, Qin LY, Chen XY, Febbraio M, Cho S.
CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury. J Neurosci. 2008 Apr 30;28(18):4661-70.
Candelario-Jalil E, Muñoz E, Fiebich BL.
Detrimental effects of tropisetron on permanent ischemic stroke in the rat. BMC Neurosci. 2008 Feb 6;9:19.
Terao S, Yilmaz G, Stokes KY, Ishikawa M, Kawase T, Granger DN.
Inflammatory and injury responses to ischemic stroke in obese mice. Stroke. 2008 Mar;39(3):943-50.
Varga-Szabo D, Braun A, Kleinschnitz C, Bender M, Pleines I, Pham M, Renné T, Stoll G, Nieswandt B.
The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction. J Exp Med. 2008 Jul 7;205(7):1583-91.
Maysami S, Lan JQ, Minami M, Simon RP.
Proliferating progenitor cells: a required cellular element for induction of ischemic tolerance in the brain. J Cereb Blood Flow Metab. 2008 Jun;28(6):1104-13.
Atochin DN, Wang A, Liu VW, Critchlow JD, Dantas AP, Looft-Wilson R, Murata T, Salomone S, Shin HK, Ayata C, Moskowitz MA, Michel T, Sessa WC, Huang PL.
The phosphorylation state of eNOS modulates vascular reactivity and outcome of cerebral ischemia in vivo. J Clin Invest. 2007 Jul;117(7):1961-7.
Tsubokawa T, Jadhav V, Solaroglu I, Shiokawa Y, Konishi Y, Zhang JH.
Lecithinized superoxide dismutase improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats.Stroke. 2007 Mar;38(3):1057-62. .
Zhang Z, Yang X, Zhang S, Ma X, Kong J.
BNIP3 upregulation and EndoG translocation in delayed neuronal death in stroke and in hypoxia.Stroke. 2007 May;38(5):1606-13.
Sayeed I, Wali B, Stein DG.
Progesterone inhibits ischemic brain injury in a rat model of permanent middle cerebral artery occlusion. Restor Neurol Neurosci. 2007;25(2):151-9.
Kleinschnitz C, Pozgajova M, Pham M, Bendszus M, Nieswandt B, Stoll G.
Targeting Platelets in Acute Experimental Stroke. Impact of Glycoprotein Ib, VI, and IIb/IIIa Blockade on Infarct Size, Functional Outcome, and Intracranial Bleeding. Circulation. 2007 May;115(17):2323-30.
Pignataro G, Simon RP, Xiong ZG.
Prolonged activation of ASIC1a and the time window for neuroprotection in cerebral ischaemia. Brain. 2007 Jan;130(Pt 1):151-8.
Pignataro G, Studer FE, Wilz A, Simon RP, Boison D.
Neuroprotection in ischemic mouse brain induced by stem cell-derived brain implants. J Cereb Blood Flow Metab. 2007 May;27(5):919-27.
Cho S, Szeto HH, Kim E, Kim H, Tolhurst AT, Pinto JT.
A novel cell-permeable antioxidant peptide, SS31, attenuates ischemic brain injury by down-regulating CD36. J Biol Chem. 2007 Feb 16;282(7):4634-42.
Pignataro G, Simon RP, Boison D.
Transgenic overexpression of adenosine kinase aggravates cell death in ischemia. J Cereb Blood Flow Metab. 2007 Jan;27(1):1-5.
Kleinschnitz C, Stoll G, Bendszus M, Schuh K, Pauer HU, Burfeind P, Renné C, Gailani D, Nieswandt B, Renné T.
Targeting coagulation factor XII provides protection from pathological thrombosis in cerebral ischemia without interfering with hemostasis. J Exp Med. 2006 Mar 20;203(3):513-8. Epub 2006 Mar 13.
Solaroglu I, Tsubokawa T, Cahill J, Zhang JH.
Anti-apoptotic effect of granulocyte-colony stimulating factor after focal cerebral ischemia in the rat. Neuroscience. 2006 Dec 28;143(4):965-74.
Shimamura N, Matchett G, Tsubokawa T, Ohkuma H, Zhang J.
Comparison of silicone-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model. J Neurosci Methods. 2006 Sep 30;156(1-2):161-5.
Khan M, Jatana M, Elango C, Paintlia AS, Singh AK, Singh I.
Cerebrovascular protection by various nitric oxide donors in rats after experimental stroke. Nitric Oxide. 2006 Sep;15(2):114-24.
Shimamura N, Matchett G, Yatsushige H, Calvert JW, Ohkuma H, Zhang J.
Inhibition of integrin alphavbeta3 ameliorates focal cerebral ischemic damage in the rat middle cerebral artery occlusion model. Stroke. 2006 Jul;37(7):1902-9.
Liu S, Liu W, Ding W, Miyake M, Rosenberg GA, Liu KJ.
Electron paramagnetic resonance-guided normobaric hyperoxia treatment protects the brain by maintaining penumbral oxygenation in a rat model of transient focal cerebral ischemia. J Cereb Blood Flow Metab. 2006 Oct;26(10):1274-84.
Tsubokawa T, Yamaguchi-Okada M, Calvert JW, Solaroglu I, Shimamura N, Yata K, Zhang JH.
Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats. J Neurosci Res. 2006 Sep;84(4):832-40.
Tsubokawa T, Solaroglu I, Yatsushige H, Cahill J, Yata K, Zhang JH.
Cathepsin and calpain inhibitor E64d attenuates matrix metalloproteinase-9 activity after focal cerebral ischemia in rats. Stroke. 2006 Jul;37(7):1888-94.