Extended Middle Cerebral Artery Occlusion (MCAO) Model to Mirror Stroke Patients Undergoing Thrombectomy

SJ Messmer, KE Salmeron, JA Frank… - Translational Stroke …, 2021 - Springer
… Corporation’s recommendations on filament size based on weight did not fit with our animal
model, but the Doccol filaments were ideal for our surgery based on their gentle approach
with a silicone-coated tip. For the 5t-MCAO surgery model, we determined that for female rats …

… mouse cage warming system provides improved intra-ischemic and post-ischemic body temperature control–application for reducing variability in experimental stroke

SH Hong, JH Hong, MT Lahey, L Zhu… - Journal of Neuroscience …, 2021 - Elsevier
… Our results support the benefit of tighter body temperature control for reducing variability of
stroke outcome measures. Lastly, we describe … Two occluder filaments were used to achieve
the mild (0.21 mm diam, 2-3 mm coating length, 602123PK10Re, Doccol) or severe stroke

[PDF][PDF] Gut Microbial Dysbiosis is Correlated with Stroke Severity Markers in Aged Rats Following Stroke

TC Hammond, S Messmer, JA Frank, D Lukins… - 2021 - researchsquare.com
… Recent advances in 64 acute stroke therapies have lowered stroke mortality, but survivors
are often left severely 65 impaired2. Rehabilitation therapies are beneficial at inducing …
exception that Doccol Corporation silicone rubber-coated monofilaments were used for the 145 …

[HTML][HTML] Infarct growth precedes cerebral thrombosis following experimental stroke in mice

V Göb, MG Voll, L Zimmermann, K Hemmen, G Stoll… - Scientific Reports, 2021 - nature.com
… A silicon rubber-coated filament (6021PK10, Doccol, Redlands, CA) was inserted in the carotid
artery and advanced up to the origin of the middle cerebral artery (MCA). Surgical procedure
was kept below 10 min for each mouse. After 60 min, the filament was removed allowing …

MicroRNA miR-21 Decreases Post-stroke Brain Damage in Rodents

MS Lopez, KC Morris-Blanco, N Ly, C Maves… - Translational Stroke …, 2021 - Springer
… In brief, mice were anesthetized with isoflurane, and MCAO was conducted using an intraluminal
6–0 silicone-coated nylon monofilament suture (Doccol, USA). Following a 1 h occlusion,
the suture was withdrawn to restore the blood flow. Physiological parameters (pH, PaO 2 , …

Post-acute delivery of α5-GABAA antagonist, S 44819, improves functional recovery in juvenile rats following stroke

F Lebrun, N Violle, A Letourneur, C Muller… - Experimental …, 2022 - Elsevier
… It is why we decided to start S 44819 treatment at D3 after stroke to avoid interference during
acute phase of stroke. Based on this scientific … Firstly we had to determine the silicone-coated
monofilament (Doccol, Sharon, MA, USA) by comparing 3 following diameters: 0.27, 0.29 …

[HTML][HTML] Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice

Y Wang, H Jin, Y Wang, Y Yao, C Yang, J Meng… - Theranostics, 2021 - ncbi.nlm.nih.gov
… Focal cerebral ischemia was induced by 45 min of transient middle cerebral artery occlusion
(MCAo) by inserting a silicone-coated 6-0 monofilament (Doccol Corp, CA, USA) into the left
common carotid artery to block the MCA, as previously reported 18 . Sham-operated mice …

Physical therapy exerts sub-additive and suppressive effects on intracerebral neural stem cell implantation in a rat model of stroke

H Ghuman, N Perry, L Grice, M Gerwig… - Journal of Cerebral …, 2021 - journals.sagepub.com
… study will guide and inform the incorporation of PT in the design of clinical trials evaluating
intraparenchymal NSCs implantation for stroke. … Multi-modal PT, including resistance
training, aerobic exercise (AE) and task integration, is considered the standard of care for stroke

[HTML][HTML] Extracellular vesicles from hypoxia-preconditioned microglia promote angiogenesis and repress apoptosis in stroke mice via the TGF-β/Smad2/3 pathway

L Zhang, W Wei, X Ai, E Kilic, DM Hermann… - Cell death & …, 2021 - nature.com
… Motor coordination was evaluated using the rotarod test (D), balance beam test (E),
tightrope test (F), corner turn test (G), and paw slips recording (H) 1 day before stroke (pre) as
well as 2, 5 and 7 days after stroke. All animals were accordingly trained before the induction of …

Imperatorin inhibits mitogen‐activated protein kinase and nuclear factor kappa‐B signaling pathways and alleviates neuroinflammation in ischemic stroke

J Ge, S Deng, Z Xue, P Liu, L Yu, J Li… - CNS Neuroscience …, 2021 - Wiley Online Library
… A piece of 6/0 monofilament nylon suture with a heat- rounded tip (Doccol corporation, MA,
USA) was inserted into ECA and arranged to obstruct the origin of MCA. Laser Doppler flowmetry
(Perimed corporation, Stockholm, Sweden) was used to assess the cerebral blood flow. …